Apl case study

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Case Study The identifying chromosomal abnormality related with APL is t (15;17) which is identified stylish ~90% of cases. This translocation results in the coalition of promyelocytic leukaemia (PML) and the retinoic acid sensory receptor alpha (RARA) protein.

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How is qPCR used in acute promyelocytic leukemia?

Because the classic t (15;17) translocation creates two fusion genes from one splice, 70% of cases with translocation, harbor a RARA/PML gene, and 100% show the reciprocal PML/RARA gene. 2 This makes qPCR a useful assay for disease screening. Treatment of acute promyelocytic leukemia is unique and involves a combination regimen.

How are cases of apl confirmed in the laboratory?

Cases of APL with t (15;17) are confirmed through laboratory testing and bone marrow observations combined with molecular techniques. Cellular antigenic profiles and chromosomal and genetic mapping are important in determining cells that have the abnormal fusion transcript.

What are the survival rates for APL patients?

The PML/RARA or variant version of a RARA fusion protein not only blocks binding of retinoic acid to its receptor but also activates genes that aid in cell self-renewal. 1,2 Survival rates for patients diagnosed with APL with t (15; 17) are good.

Last Update: Oct 2021

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